TREVIGEN代理-萊恩生物科技有限公司。活細胞染劑Calcein AM，細胞增殖分析及觀察。配合10倍dissociation溶液，螢光定量癌細胞migration及 invasion。

Calcein AM為非螢光染劑，Calcein(鈣黃綠素)的基礎上加強了疏水性，因此能夠輕易穿透活細胞膜。當其進入細胞質後，被細胞中的酯酶水解為Calcein，並發出強綠色螢光物質。Calcein AM細胞毒性低，因此為為細胞螢光染色最佳選擇。Calcein AM不會抑制任何的細胞功能如增殖或淋巴球的趨化性，與標準的51Cr-釋放法所得結果相一致的。

Calcein AM可應用於活細胞觀察及cell proliferation分析、活細胞和死細胞的雙重染色、Endothelial cell migration、Endothelial cell tubulogenesis、Tumor cell invasion 細胞侵入試驗。

原廠連結: www.trevigen.com
 

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

研究Apoptosis-測定ADP/ATP比例的改變,可以區別細胞死亡或存活比例。BIOVISION代理-萊恩生物科技有限公司。

產品特點:

同時偵測細胞凋亡(apoptosis)細胞壞死(necosis) 細胞休止(cell arrest)及

細胞增殖(cell proliferation)

靈敏度高 100 c ells/well

操作簡單 快速且具高效率

用途: 測定ADP/ATP比例的改變,來區別細胞死亡或存活

例如: proliferating cells: ATP增加 /ADP減少

Apoptotic cell: ATP減少/ADP增加

Necrosis cell: ATP顯著減少/ADP顯著增加

Cell Fate	ADP Level	ATP Level	ADP/ATP
Proliferation	Very Low	High	Very Low
Growth Arrest	Low	Slightly Increased	Low
Apoptosis	High	Low	High
Necrosis	Much Higher	Very Low	Much Higher

實驗方法:

(一) 將懸浮細胞取10ul (10³~10⁴)至白盤並加入100 μl Nucleotide Releasing Buffer

或貼附型細胞suction培養液後,取10³~10⁴加入100 μl Nucleotide Releasing Buffer,於室溫輕

輕反轉混合5分

(二) 加入1ul ATP Monitoring Enzyme至上述已準備好的cell lysate中, 1分鐘後以冷光儀測量細胞中ATP量(data A)

(三) 同樣的sample 於10分鐘後測量細胞中ADP量(data B)

(四) 再加入1 μl 的 ADP Converting Enzyme.並1分鐘後再測量一次(data C)

計算ADP/ATP的ratio₌ Data C – Data B /Data A

原廠連結: http://www.biovision.com/

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

發現HDAC，新一代明星標靶抗癌藥物之ㄧ，透過改變癌細胞的生長週期,停止癌細胞生長，抑制癌細胞生長、分化。BIOVISON代理商-太鼎生物科技有限公司。

[「HDAC 抑制劑」被視為新一代明星標靶抗癌藥物之ㄧ,主要作用機轉是透過改變癌細胞的生長週期,停止癌細胞生長、誘導癌細胞分化為正常細胞、或是造成癌細胞凋零死亡,來達到治療癌症效果]。Bivision提供多種分析HDAC的Kit, HDAC抑制劑, HDAC抗體.

HDAC 分析/藥物篩選

K331-100 HDAC 呈色分析套組 100 assays

K 333-11-30 HDAC Family 抗體套組 11 antibodies

K330-100 HDAC 螢光分析套組 100 assays

K340-100 HDAC Inhibitor 藥物篩選套組 100 assays

HDAC 抑制劑

1606-1 Trichostatin A抑制劑 1 mg

1604-1 Vorinostat/SAHA 抑制劑 1 mg

1607 -1 M 344 抑制劑 1 mg

1608-100 Sodium 4-phenylbutyrate 抑制劑 100 mg

1608-1000 Sodium 4-phenylbutyrate 抑制劑 1 gram

1609-1000 Sodium butyrate 抑制劑 1 g

1610-5 Splitomicin 抑制劑 5 mg

1606-1 Trichostatin A 抑制劑 1 mg

1601-1 Apicidin 抑制劑 1 mg

K851-6 DiscoveryPak™ HDAC 抑制劑套組 6 Inhibitors

原廠連結: http://www.biovision.com/
 

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com

【磷酸化分析】PiColorLock採用超高靈敏度Malachite green磷酸根呈色法。測量ATPases、phosphatases、GTPases酵素活性。innovabiosciences。

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com
 

【如何建立快速藥物篩選系統】

PiColorLock採用超高靈敏度Malachite green磷酸根呈色方法，克服傳統呈色方法,低靈敏度限制。測量目標樣本中，是否含有ATPases、phosphatases、GTPases、nucleotidases酵素活性。

inorganic phosphate detection

產品應用

Innova Biosciences PiColorLock 針對實驗初篩所設計，可探討酵素活性是否扮演transport、signal transduction, protein biosynthesis及cell differentiation之角色。應用於ATPase、GTPase、Proteinases活性分析。提供high-throughput screen 大量ATPase或GTPase inhibitor。

PiColorLock產品，有分為Gold (靈敏度4~40uM)及ALS(靈敏度20~200uM)。

inorganic phosphate detection

產品特點

1. PiColorLock分析套組，包括stabilizing solution，可避開acid labile substrate所造成的高背景值，其背景值很低( 1mM )。

2. 其分析波段為600~660nm，避開一般colored compounds之干擾。

3. Pi-dye複合物極為穩定，其訊號強度可維持數小時之久。

4. Superior呈色系統。採用Malachite Green磷酸根呈色方式，呈色靈敏度比傳統呈色方法高，克服低靈敏度的限制。

5. 適用於大部份的assay buffer。

6. 蛋白質濃度高時，不會抑制顏色的形成。

High Throughput Colorimetric ATPase Assays

This non radioactive colorimetric assay kit uses a 96 well format, and all the necessary reagents are supplied for measuring enzyme activity and are ideal for high throughput drug screening.

High Throughput Colorimetric GTPase Assays

This non radioactive colorimetric assay kit uses a 96 well format, and all the necessary reagents are supplied for measuring enzyme activity and are ideal for high throughput drug screening.

Pi Colorlock (ALS)

PiColorLock is a phosphate detection reagent for measuring Phosphatases, ATPases and Nucleotidases.

Pi Colorlock (Gold)

PiColorLock is a phosphate detection reagent for measuring Phosphatases, ATPases and Nucleotidases.

Colorimetric assays for ATPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions. Such assays are beset with problems of reagent precipitation and high backgrounds caused by impure substrates (i.e. contaminated with inorganic phosphate) and/or by non-enzymatic (acid) hydrolysis of the substrate.

At the heart of our assay kits is PiColorLock Gold, a superior phosphate detection reagent which ensures high stability of the colored dye-phosphate complexes. Together with specially purified substrates and proprietary stabilizers our kits offer the lowest possible assay backgrounds and outstanding assay performance.

Uniquely, Innova Biosciences ATPase assay kits contain highly purified ATP, which have had any free inorganic phosphate removed – this means the assays have the lowest possible background signal.

PiColorLock reagent may also be purchased separately for developing simple assays for any phosphate-generating enzyme. It is offered in both high (Gold) and standard sensitivity (ALS) formulations to suit almost any assay situation.

更多參考內容:

原廠連結: http://www.innovabiosciences.com/

文獻發表

ATPase Assay Kit

View Product

• Caveolin-1 regulates proliferation and osteogenic differentiation of human mesenchymal stem cells- Journal of Cellular Biochemistry (2012)
• Common Pathogenic Effects of Missense Mutations in the P-Type ATPase ATP13A2 (PARK9) Associated with Early-Onset Parkinsonism - PLOS One (2012)
• Structure of the VirB4 ATPase, alone and bound to the core complex of a type IV secretion system - PNAS (2012)
• Peroxisomal proteostasis involves a Lon family protein that functions as protease and chaperone - JBC (2012)
• Xeroderma pigmentosum group F protein binds to Eg5 and is required for proper mitosis: implications for XP-F and XFE- Genes to Cells (2012)
• hSWS1·SWSAP1 Is an Evolutionarily Conserved Complex Required for Efficient Homologous Recombination Repair - JBC (2011)
• Enzyme Stability of Microencapsulated Bifidobacterium animalis ssp. lactis Bb12 after Freeze Drying and during Storage in Low Water Activity at Room Temperature - Journal of Food Science (2011)
• Digitoxin and a synthetic monosaccharide analog inhibit cell viability in lung cancer cells - Toxicology and Applied Pharmacology (2011)
• Functional defect caused by the 4544G>A SNP in ABCC2: potential impact for drug cellular disposition - Pharmacogenetics and Genomics (2011)
• A Common Variant in TFB1M Is Associated with Reduced Insulin Secretion and Increased Future Risk of Type 2 Diabetes - Cell Metabolism (2011)
• Overexpression of the Endoplasmic Reticulum Chaperone BiP3 Regulates XA21-Mediated Innate Immunity in Rice– PLoS One (2010)
• Identification of gp96 as a Novel Target for Treatment of Autoimmune Disease in Mice - PLoS One (2010)
• Antimicrobial Mechanism of Action of Transferrins: Selective Inhibition of H⁺-ATPase - Antimicrob Agents Chemother. (2010)
• Modulation of UvrD Helicase Activity by Covalent DNA-Protein Cross-links - Biol Chem. (2010)
• Monoclonal antibodies against nucleoside triphosphate hydrolase-II can reduce the replication of Toxoplasma gondii - Parasitology (2010)
• Biochemical Dissection of the ATPase TraB, the VirB4 Homologue of theEscherichia coli pKM101 Conjugation Machinery - J. Bacteriol. (2010)
• Identification of gp96 as a Novel Target for Treatment of Autoimmune Disease in Mice– PLoS One (2010)
• ATP-binding cassette-like transporters are involved in the transport of lignin precursors across plasma and vacuolar membranes - PNAS (2010)
• Arabidopsis stromal 70-kDa heat shock proteins are essential for chloroplast development - Planta (2010)
• Lipopeptide induces apoptosis in fungal cells by a mitochondria-dependent pathway - Peptides (2010)
• Modulation of a Schistosoma mansoni multidrug transporter by the antischistosomal drug praziquantel - The FASEB Journal (2010)
• Biosynthesis of (Bacterio)chlorophylls ATP-Dependent Transient Subunit Interaction And Electron Transfer Of Dark Operative Protochlorophyllide Oxireductase - JBC (2010)
• Stress-Dependent Coordination of Transcriptome and Translatome in Yeast - PLoS Biology (2009)
• Crystal Structure of a Novel Conformational State of the Flavivirus NS3 Protein: Implications for Polyprotein Processing and Viral Replication - J Virol (2009)
• ATP and MO25α Regulate the Conformational State of the STRADα Pseudokinase and Activation of the LKB1 Tumour Suppressor – PLoS Biol (2009)
• Apratoxin A Reversibly Inhibits the Secretory Pathway by Preventing Cotranslational Translocation - Molecular Pharmacology (2009)
• NS4A regulates the ATPase activity of the NS3 helicase: a novel cofactor role of the non-structural protein NS4A from West Nile virus - Journal of General Virology (2009)
• Evidence That Two ATP-Dependent (Lon) Proteases in Borrelia burgdorferi Serve Different Functions - PLoS Pathogens (2009)
• ATP and MO25α Regulate the Conformational State of the STRADα Pseudokinase and Activation of the LKB1 Tumour Suppressor- PLoS Biology (2009)
• Stress-Dependent Coordination of Transcriptome and Translatome in Yeast - PLoS Biology (2009)
• An Afg2/Spaf-Related Cdc48-like AAA ATPase Regulates the Stability and Activity of the C. elegans Aurora B Kinase AIR-2 - Developmental Cell (2008)
• Staphylococcus aureus HrtA Is an ATPase Required for Protection against Heme Toxicity and Prevention of a Transcriptional Heme Stress Response - Journal of Bacteriology (2008)
• ATP scavenging by the intracellular pathogen Porphyromonas gingivalis inhibits P2X₇-mediated host-cell apoptosis - Cell Microbiol. (2008)
• The Two-component NS2B-NS3 Proteinase Represses DNA Unwinding Activity of the West Nile Virus NS3 Helicase - JBC (2008)
• Functional characterization of the nucleotide binding domain of the Cryptosporidium parvum CpABC4 transporter: An iron–sulfur cluster transporter homolog - Molecular and Biochemical Parasitology (2008)
• Hippocampal levels of gamma-enolase, C-1-tetrahydrofolate synthase and serotransferrin fluctuate over the estrous cycle in the rat - Neuroscience (2008)
• Consumption of Soy Protein Isolate Modulates the Phosphorylation Status of Hepatic ATPase/ATP Synthase ß Protein and Increases ATPase Activity in Rats - J. Nutr. (2007)
• First identification and functional characterization of an immunogenic protein in unculturable haemotrophic Mycoplasmas (Mycoplasma suis HspA1) - FEMS Immunology & Medical Microbiology (2007)
• Identification, structure and mode of action of a new regulator of the Helicobacter pylori HP0525 ATPase - EMBO J. (2007)
• Proteome Profiling in the Rat Harderian Gland - J Proteome Res (2006)

GTPase Assay Kit

View Product

• Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment - Nature Cell Biology (2012)
• The Mitochondrial Phosphatase PGAM5 Functions at the Convergence Point of Multiple Necrotic Death Pathways- Cell (2012)
• Leucyl-tRNA Synthetase Is an Intracellular Leucine Sensor for the mTORC1-Signaling Pathway - Cell (2012)
• ArfGAP1 Is a GTPase Activating Protein for LRRK2: Reciprocal Regulation of ArfGAP1 by LRRK2 - Journal of Neuroscience (2012)
• GTPase Activity and Neuronal Toxicity of Parkinson's Disease–Associated LRRK2 Is Regulated by ArfGAP1- PLoS Genetics (2012)
• AIP1 Functions as Arf6-GAP to Negatively Regulate TLR4 Signaling - JBC (2010)
• GTPase activity plays a key role in the pathobiology of LRRK2 - PLoS Genetics (2010)
• Role of the G-protein and tyrosine kinase—Rho/ROK pathways in 15-hydroxyeicosatetraenoic acid induced pulmonary vasoconstriction in hypoxic rats - J Biochem (2010)
• AIP1 Functions as Arf6-GAP to Negatively Regulate TLR4 Signaling - JBC (2010)
• Identification and Characterization of a Leucine-Rich Repeat Kinase 2 (LRRK2) Consensus Phosphorylation Motif- PLoS One (2010)
• Speckled-like Pattern in the Germinal Center (SLIP-GC), a Nuclear GTPase Expressed in Activation-induced Deaminase-expressing Lymphomas and Germinal Center B Cells- JBC (2009)
• High-level production and characterization of a G-protein coupled receptor signaling complex - FEBS Journal (2009)
• Structure of ERA in complex with the 3′ end of 16S rRNA: Implications for ribosome biogenesis- PNAS (2009)
• G Protein-Coupled Receptor-Promoted Trafficking of Gβ1γ2 Leads to AKT Activation at Endosomes via a Mechanism Mediated by Gβ1γ2-Rab11a Interaction – Molecular Biology of the Cell (2008)
• Characterization of Human GTPBP3, a GTP-Binding Protein Involved in Mitochondrial tRNA Modification - Molecular and Cellular Biology (2008)
• Comparative real-time PCR and enzyme analysis of selected gender-associated molecules in Schistosoma japonicum - Parasitology (2008)

PiColorlock Gold

View Product

• Kinetic Modelling of GlmU Reactions – Prioritization of Reaction for Therapeutic Application- PLoS One (2012)
• The unusual mycobacterial chaperonins: evidence for in vivo oligomerization and specialization of function- Molecular Microbiology (2012)
• Synthesis and Antibacterial Evaluation of a New Series of N-Alkyl-2-alkynyl/(E)-alkenyl-4-(1H)-quinolones- Molecules (2012)
• PPM1H Is a p27 Phosphatase Implicated in Trastuzumab Resistance - Cancer Discovery (2011)
• Sepsis impairs alveolar epithelial function by downregulating Na-K-ATPase pump - AJP - Lung Physiol (2011)
• Structural and functional insights into DNA-end processing by the archaeal HerA helicase–NurA nuclease complex - Nucleic Acids Research (2011)
• Sepsis impairs alveolar epithelial function by downregulating Na-K-ATPase pump- AJP - Lung Physiol (2011)
• FXYD Proteins Stabilize Na,K-ATPase, Amplification Of Specific Phosphatidylserine -Protein Interactions- JBC (2011)
• Structural and functional insights into DNA-end processing by the archaeal HerA helicase–NurA nuclease complex - Nucl Acids Res (2011)
• Stabilization of the α2 isoform of Na,K-ATPase by mutations in a phospholipid binding pocket - JBC (2011)
• Characterisation of multiple substrate-specific (d)ITP/(d)XTPase and modelling of deaminated purine nucleotide metabolism - MIMS EPrints (2011)
• Substrate uptake, phosphorus repression, and effect of seed culture on glycopeptide antibiotic production: Process model development and experimental validation - Biotechnology and Bioengineering (2010)
• Selectivity of Digitalis Glycosides for Isoforms of Human Na,K-ATPase- JBC (2010)
• ATP-dependent MurE ligase in Mycobacterium tuberculosis: Biochemical and structural characterisation - Tuberculosis (2010)
• <a href="http://www.jbc.org</body></html>

Innova Biosciences PiColorLock 針對實驗初篩所設計，應用於ATPase、GTPase、Proteinases活性分析。

Innova Biosciences PiColorLock 針對實驗初篩所設計，可探討酵素活性是否扮演transport、signal transduction, protein biosynthesis及cell differentiation之角色。應用於ATPase、GTPase、Proteinases活性分析。提供high-throughput screen 大量ATPase或GTPase inhibitor。

【供應廠商】萊恩生物科技有限公司

【公司電話】04-2523-9639

【業務專員】陳俊豪 0937-229803

【電子信箱】biolion0819@gmail.com