HDAC Assay Kits & Reagents
HDACs牽涉轉錄調控，並誘導細胞凋亡或癌細胞之分化。Biovision提供螢光及呈色分析套組，操作簡單且快速，方便測量樣品中的HDAC活性。此方式排除放射性標誌，萃取等傳統分析。
Inhibition of histone deacetylases (HDACs) has been implicated to modulate transcription and to induce apoptosis or differentiation in cancer cells. The HDAC Fluorometric and Colorimetric Assay Kits provide simple procedures for fast and convenient measurement of HDAC activity by fluorometric and colorimetric methods. The methods eliminate radioactivity, extractions, or chromatography, as used in the traditional assays. The new procedures require only two easy steps, both performed on the same microtiter plate. First, the HDAC fluorometric (or colorimetric) substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (e.g., HeLa nuclear extract). Deacetylation sensitizes the substrate, and thus in the second step, treatment with the Lysine Developer produces a fluorophore (or chromophore), which can be analyzed using a fluorometric (or colorimetric) plate reader. The HDAC Inhibitor Drug Screening Kit provides sufficient positive controls for screening 100 samples of HDAC inhibitor candidates in drug discovery. HDAC inhibitors and antibodies are also available separately.
【HDAC於腫瘤形成之重要性】
組蛋白的乙醯化修飾對腫瘤的發生發展起重要作用。正常細胞體一旦出現核內組蛋白乙醯化與去乙醯化的失衡，即會導致正常的細胞週期與細胞代謝行為的改變而誘發腫瘤。組蛋白去乙醯化酶(histone deacetylases，HDACs)催化組蛋白的去乙醯化，維繫組蛋白乙醯化與去乙醯化的平衡狀態，與癌相關基因轉錄表達、細胞增殖分化及細胞凋亡等諸多過程密切相關。組蛋白去乙醯化酶(histone deacetylases，HDACs)是維持染色體的基本組成單位核小體中組蛋白乙醯化平衡的關鍵酶類之一，其催化組蛋白的去乙醯化作用，與基因轉錄抑制密切相關，牽涉到促基因沉默的諸多過程，是抗腫瘤藥物設計中的熱門靶標。
HDAC Assay Kits
|
Cat#
|
Size
|
HDAC Fluorometric Assay Kit
|
K330-100
|
100 assays
|
HDAC Colorimetric Assay Kit
|
K331-100
|
100 assays
|
HDAC Inhibitor Drug Screening Kit
|
K340-100
|
100 assays
|
HDAC Enzymes
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Cat#
|
Size
|
HeLa Nuclear Extract
|
1641-01
|
100 ug
|
HeLa Nuclear Extract
|
1641-1
|
1 mg
|
Recombinant HAT Protein
|
1137-100
|
100 ug
|
HDAC Inhibitors
|
Cat#
|
Size
|
Apicidin
|
1601-1
|
1 mg
|
M344
|
1607-1
|
1 mg
|
MS-275 (Entinostat, SNDX-275)
|
1590-1
|
1 mg
|
MS-275 (Entinostat, SNDX-275)
|
1590-5
|
5 mg
|
Panobinostat (LBH589)
|
1612-1
|
1 mg
|
Panobinostat (LBH589)
|
1612-5
|
5 mg
|
Sodium 4-phenylbutyrate
|
1608-100
|
100 mg
|
Sodium 4-phenylbutyrate
|
1608-1000
|
1 gram
|
Sodium butyrate
|
1609-1000
|
1 g
|
Splitomicin
|
1610-5
|
5 mg
|
Trichostatin A
|
1606-1
|
1 mg
|
Valproic Acid, Sodium Salt
|
1647-200
|
200 mg
|
HDAC Inhibitor Set
|
Cat#
|
Size
|
DiscoveryPakTM HDAC Inhibitor Set
|
K851-6
|
6 inhibitors
|
HDAC Antibodies
|
Cat#
|
Size
|
Histone Deacetylase 1 (HDAC1) pAb
|
3601-100
|
100 ug
|
Histone Deacetylase 2 (HDAC2) pAb
|
3602-100
|
100 ug
|
Histone Deacetylase 3 (HDAC3) pAb
|
3603-100
|
100 ug
|
Histone Deacetylase 4 (HDAC4) pAb
|
3604-100
|
100 ug
|
Histone Deacetylase 4 (HDAC4) pAb
|
3604A-100
|
100 ug
|
Histone Deacetylase 5 (HDAC5) pAb
|
3605-100
|
100 ug
|
Histone Deacetylase 6 (HDAC6) pAb
|
3606-100
|
100 ug
|
Histone Deacetylase 7 (HDAC7) pAb
|
3607-100
|
100 ug
|
Histone Deacetylase 8 (HDAC8) pAb
|
3608-100
|
100 ug
|
Histone Deacetylase 9 (HDAC9) pAb
|
3609-100
|
100 ug
|
Histone Deacetylase 10 (HDAC10) pAb
|
3610-100
|
100 ug
|
Histone Deacetylase 11 (HDAC11) pAb
|
3611P-100
|
100 ug
|
Histone H 2A pAb
|
3621-100
|
100 ug
|
Histone H2B pAb
|
3622-100
|
100 ug
|
Histone H3 pAb
|
3623-100
|
100 ug
|
Histone H4 pAb
|
3624-100
|
100 ug
|
HDAC Family Antibody Set
|
K 333-11-30
|
Set of 11
|
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