PiColorLock磷酸根呈色----產品介紹
產品應用於ATPases、phosphatases、GTPases、nucleotidases酵素活性之分析。
PiColorLock採用超高靈敏度Malachite green磷酸根呈色方法，克服傳統呈色方法,低靈敏度限制。測量目標樣本中，是否含有ATPases、phosphatases、GTPases、nucleotidases酵素活性。
inorganic phosphate detection
產品應用
Innova Biosciences PiColorLock 針對實驗初篩所設計，可探討酵素活性是否扮演transport、signal transduction, protein biosynthesis及cell differentiation之角色。應用於ATPase、GTPase、Proteinases活性分析。提供high-throughput screen 大量ATPase或GTPase inhibitor。可建立快速藥物篩選系統。
inorganic phosphate detection
產品特點
1. PiColorLock分析套組，包括stabilizing solution，可避開acid labile substrate所造成的高背景值，其背景值很低(1mM )。
2. 其分析波段為600~660nm，避開一般colored compounds之干擾。
3. Pi-dye複合物極為穩定，其訊號強度可維持數小時之久。
4. Superior呈色系統。採用Malachite Green磷酸根呈色方式，呈色靈敏度比傳統呈色方法高，克服低靈敏度的限制。
5. 適用於大部份的assay buffer。
6. 蛋白質濃度高時，不會抑制顏色的形成。
PiColorLock產品，有分為Gold (靈敏度4~40uM)及ALS(靈敏度20~200uM)。
訂購貨號# 302-0125, 625/1560 assays (靈敏度20~200uM)
訂購貨號# 302-0500, 2500/6250 assays (靈敏度20~200uM)
PiColorLock is a phosphate detection reagent for measuring Phosphatases, ATPases and Nucleotidases.
訂購貨號# 303-0125, 625/1560 assays (靈敏度4~40uM)
訂購貨號# 303-0500, 2500/6250 assays (靈敏度4~40uM)
PiColorLock is a phosphate detection reagent for measuring Phosphatases, ATPases and Nucleotidases.
Colorimetric assays for ATPases are invariably based on the formation of colored complexes between an inorganic phosphate and a dye molecule under acidic conditions. Such assays are beset with problems of reagent precipitation and high backgrounds caused by impure substrates (i.e. contaminated with inorganic phosphate) and/or by non-enzymatic (acid) hydrolysis of the substrate.
At the heart of our assay kits is PiColorLock Gold, a superior phosphate detection reagent which ensures high stability of the colored dye-phosphate complexes. Together with specially purified substrates and proprietary stabilizers our kits offer the lowest possible assay backgrounds and outstanding assay performance.
Uniquely, Innova Biosciences ATPase assay kits contain highly purified ATP, which have had any free inorganic phosphate removed – this means the assays have the lowest possible background signal.
PiColorLock reagent may also be purchased separately for developing simple assays for any phosphate-generating enzyme. It is offered in both high (Gold) and standard sensitivity (ALS) formulations to suit almost any assay situation.
更多參考內容:
相關商品
ATPase活性分析檢測套組
GTPase活性分析檢測套組
磷酸根移除resin
5g(501-0015)
Inorganic phosphate (Pi) is a product of many enzymatic reactions, for example, those catalysed by phosphatases and ATPases. Measurement of released Pi allows enzyme activity to be determined, but contamination of enzymes or buffers by Pi can lead to unacceptably high assay backgrounds.
PiBind™ resin provides a quick and easy way to remove contaminating Pi from buffers. The resin works over a broad range of pH values and is unaffected by many commonly used buffer additives. PiBind™ resin can also be used to remove Pi from protein samples (e.g. tissue extracts). This method is quicker than dialysis and unlike desalting does not necessarily lead to a significant dilution of the sample.
PiColorlock Gold/ALS文獻發表
PiColorlock Gold
- Kinetic Modelling of GlmU Reactions – Prioritization of Reaction for Therapeutic Application- PLoS One (2012)
- The unusual mycobacterial chaperonins: evidence for in vivo oligomerization and specialization of function- Molecular Microbiology (2012)
- Synthesis and Antibacterial Evaluation of a New Series of N-Alkyl-2-alkynyl/(E)-alkenyl-4-(1H)-quinolones- Molecules (2012)
- PPM1H Is a p27 Phosphatase Implicated in Trastuzumab Resistance - Cancer Discovery (2011)
- Sepsis impairs alveolar epithelial function by downregulating Na-K-ATPase pump - AJP - Lung Physiol (2011)
- Structural and functional insights into DNA-end processing by the archaeal HerA helicase–NurA nuclease complex - Nucleic Acids Research (2011)
- Sepsis impairs alveolar epithelial function by downregulating Na-K-ATPase pump- AJP - Lung Physiol (2011)
- FXYD Proteins Stabilize Na,K-ATPase, Amplification Of Specific Phosphatidylserine -Protein Interactions- JBC (2011)
- Structural and functional insights into DNA-end processing by the archaeal HerA helicase–NurA nuclease complex - Nucl Acids Res (2011)
- Stabilization of the α2 isoform of Na,K-ATPase by mutations in a phospholipid binding pocket - JBC (2011)
- Characterisation of multiple substrate-specific (d)ITP/(d)XTPase and modelling of deaminated purine nucleotide metabolism - MIMS EPrints (2011)
- Substrate uptake, phosphorus repression, and effect of seed culture on glycopeptide antibiotic production: Process model development and experimental validation - Biotechnology and Bioengineering (2010)
- Selectivity of Digitalis Glycosides for Isoforms of Human Na,K-ATPase - JBC (2010)
- ATP-dependent MurE ligase in Mycobacterium tuberculosis: Biochemical and structural characterisation - Tuberculosis (2010)
- Mechanism of Action of an Imidopiperidine Inhibitor of Human Polynucleotide Kinase/Phosphatase- JBC (2010)
- Sensor combination and chemometric variable selection for online monitoring of Streptomyces coelicolor fed-batch cultivations - Applied Microbiology and Biotechnology (2010)
- Roles of Electrostatics and Conformation in Protein-Crystal Interactions - PLoS One (2010)
- Anti-tubercular screening of natural products from Colombian plants: 3-methoxynordomesticine, an inhibitor of MurE ligase of Mycobacterium tuberculosis - J Antimicrob Chemother (2010)
- Oligomeric assembly and interactions within the human RuvB-like RuvBL1 and RuvBL2 complexes - Biochem. J. (2010)
- Functional study on GTP hydrolysis by the GTP-binding protein from Sulfolobus solfataricus, a member of the HflX family - J. Biochem. (2010)
- Functional study on GTP hydrolysis by the GTP-binding protein from Sulfolobus solfataricus, a member of the HflX family - J Biochem (2010)
- Identification of a Small Molecule Inhibitor of the Human DNA Repair Enzyme Polynucleotide Kinase/Phosphatase - Cancer Res (2009)
- Structural and Mutational Analyses of Deinococcus radiodurans UvrA2 Provide Insight into DNA Binding and Damage Recognition by UvrAs- Structure (2009)
- Characterization of Streptococcus gordonii SecA2 as a Paralogue of SecA - J Bacteriol (2009)
- Characterization of Human GTPBP3, a GTP-Binding Protein Involved in Mitochondrial tRNA Modification - Mol Cell Biol (2008)
- Stabilization of Na+,K+-ATPase Purified from Pichia pastoris Membranes by Specific Interactions with Lipids - Biochemistry (2007)
- Purification of the Human α2 Isoform of Na,K-ATPase Expressed in Pichia pastoris. Stabilization by Lipids and FXYD1 - Biochemistry (2007)
PiColorlock ALS
- Formation of a Stable RuvA Protein Double Tetramer Is Required for Efficient Branch Migration in Vitro and for Replication Fork Reversal in Vivo - JBC (2011)
- A Tethered Bilayer Assembled on Top of Immobilized Calmodulin to Mimic Cellular Compartmentalization- PLoS (2011)
- Oligomeric assembly and interactions within the human RuvB-like RuvBL1 and RuvBL2 complexes - Biochem J (2010)
PiBind resin
- Antimicrobial Mechanism of Action of Transferrins: Selective Inhibition of H+-ATPase- Antimicrobial Agents and Chemotherapy (2010)
- Role of the G-protein and tyrosine kinase—Rho/ROK pathways in 15-hydroxyeicosatetraenoic acid induced pulmonary vasoconstriction in hypoxic rats - JBC (2010)
- Protein phosphatase 1-dependent transcriptional programs for long-term memory and plasticity - Learn Mem (2010)
- Protein Phosphatase 1 Regulates the Histone Code for Long-Term Memory - J Neuroscience (2009)
- Consumption of Soy Protein Isolate Modulates the Phosphorylation Status of Hepatic ATPase/ATP Synthase ß Protein and Increases ATPase Activity in Rats - J. Nutr. (2007)
- Parvalbumin deficiency in fast-twitch muscles leads to increased ‘slow-twitch type’ mitochondria, but does not affect the expression of fiber specific proteins - FEBS Journal (2006)
【供應廠商】太鼎生物科技有限公司
【連絡人】許虹宜0920-312382
【臺北公司】02-86609496
【台中公司】陳俊豪 0935-229803
【公司網址】 www.biopioneer.com.tw
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