ATPase分析套組---產品介紹
產品應用ATPases酵素活性之分析
內附有ATPase inhibitor。ATPase酵素活性分析，採用超高靈敏度Malachite green磷酸根呈色方法，克服傳統呈色方法,低靈敏度限制。測量目標樣本中，ATPases酵素活性。
ATPase活性分析檢測
產品應用
Innova Biosciences 提供之ATPase分析套組，可探討酵素活性是否扮演transport、signal transduction, protein biosynthesis及cell differentiation之角色。應用於ATPase活性分析。提供high-throughput screen 大量ATPase inhibitor。可建立快速藥物篩選系統。
ATPase活性分析檢測
產品特點
1. 提供high-throughput screen 大量ATPase inhibitor。
2. ATPase酵素分析套組，包括stabilizing solution，可避開acid labile substrate所造成的高背景值，其背景值很低(1mM )。
2. 其分析波段為600~660nm，避開一般colored compounds之干擾。
3. Pi-dye複合物極為穩定，其訊號強度可維持數小時之久。
4. Superior呈色系統。採用Malachite Green磷酸根呈色方式，呈色靈敏度比傳統呈色方法高，克服低靈敏度的限制。
5. 適用於大部份的assay buffer。
6. 蛋白質濃度高時，不會抑制顏色的形成。
訂購貨號# 601-0120, 196 assays (靈敏度4~40uM)
訂購貨號# 601-0121, 480 assays (靈敏度4~40uM)
訂購貨號# 601-0122, 480*5 assays (靈敏度4~40uM)
產品內容物
1 x 10ml of PiColorLock™ Gold* (1 x 25ml)
1 x 0.25ml of Accelerator (1 x 0.5ml)
1 x 5ml of Stabiliser (1 x 10ml)
1 x 1.5ml of 0.1M MgCl2 (2 x 1.5ml)
1 x 5ml of 0.5M Tris pH 7.5 (1 x 10ml)
1 x 5ml of 0.1mM Pi standard (1 x 10ml)
更多參考內容:
相關商品
PiColorLock磷酸根分析檢測套組
GTPase活性分析檢測套組
游離磷酸根移除resin
5g(501-0015)
Inorganic phosphate (Pi) is a product of many enzymatic reactions, for example, those catalysed by phosphatases and ATPases. Measurement of released Pi allows enzyme activity to be determined, but contamination of enzymes or buffers by Pi can lead to unacceptably high assay backgrounds.
PiBind™ resin provides a quick and easy way to remove contaminating Pi from buffers. The resin works over a broad range of pH values and is unaffected by many commonly used buffer additives. PiBind™ resin can also be used to remove Pi from protein samples (e.g. tissue extracts). This method is quicker than dialysis and unlike desalting does not necessarily lead to a significant dilution of the sample.
ATPase酵素活性分析套組---文獻發表
ATPase酵素活性分析套組
ATPase Assay Kit
- Caveolin-1 regulates proliferation and osteogenic differentiation of human mesenchymal stem cells- Journal of Cellular Biochemistry (2012)
- Common Pathogenic Effects of Missense Mutations in the P-Type ATPase ATP13A2 (PARK9) Associated with Early-Onset Parkinsonism - PLOS One (2012)
- Structure of the VirB4 ATPase, alone and bound to the core complex of a type IV secretion system- PNAS (2012)
- Peroxisomal proteostasis involves a Lon family protein that functions as protease and chaperone - JBC (2012)
- Xeroderma pigmentosum group F protein binds to Eg5 and is required for proper mitosis: implications for XP-F and XFE- Genes to Cells (2012)
- hSWS1·SWSAP1 Is an Evolutionarily Conserved Complex Required for Efficient Homologous Recombination Repair - JBC (2011)
- Enzyme Stability of Microencapsulated Bifidobacterium animalis ssp. lactis Bb12 after Freeze Drying and during Storage in Low Water Activity at Room Temperature - Journal of Food Science (2011)
- Digitoxin and a synthetic monosaccharide analog inhibit cell viability in lung cancer cells - Toxicology and Applied Pharmacology (2011)
- Functional defect caused by the 4544G>A SNP in ABCC2: potential impact for drug cellular disposition - Pharmacogenetics and Genomics (2011)
- A Common Variant in TFB1M Is Associated with Reduced Insulin Secretion and Increased Future Risk of Type 2 Diabetes - Cell Metabolism (2011)
- Overexpression of the Endoplasmic Reticulum Chaperone BiP3 Regulates XA21-Mediated Innate Immunity in Rice– PLoS One (2010)
- Identification of gp96 as a Novel Target for Treatment of Autoimmune Disease in Mice - PLoS One (2010)
- Antimicrobial Mechanism of Action of Transferrins: Selective Inhibition of H+-ATPase- Antimicrob Agents Chemother. (2010)
- Modulation of UvrD Helicase Activity by Covalent DNA-Protein Cross-links- Biol Chem. (2010)
- Monoclonal antibodies against nucleoside triphosphate hydrolase-II can reduce the replication of Toxoplasma gondii - Parasitology (2010)
- Biochemical Dissection of the ATPase TraB, the VirB4 Homologue of theEscherichia coli pKM101 Conjugation Machinery - J. Bacteriol. (2010)
- Identification of gp96 as a Novel Target for Treatment of Autoimmune Disease in Mice– PLoS One (2010)
- ATP-binding cassette-like transporters are involved in the transport of lignin precursors across plasma and vacuolar membranes- PNAS (2010)
- Arabidopsis stromal 70-kDa heat shock proteins are essential for chloroplast development - Planta (2010)
- Lipopeptide induces apoptosis in fungal cells by a mitochondria-dependent pathway - Peptides (2010)
- Modulation of a Schistosoma mansoni multidrug transporter by the antischistosomal drug praziquantel - The FASEB Journal (2010)
- Biosynthesis of (Bacterio)chlorophylls ATP-Dependent Transient Subunit Interaction And Electron Transfer Of Dark Operative Protochlorophyllide Oxireductase - JBC (2010)
- Stress-Dependent Coordination of Transcriptome and Translatome in Yeast - PLoS Biology (2009)
- Crystal Structure of a Novel Conformational State of the Flavivirus NS3 Protein: Implications for Polyprotein Processing and Viral Replication - J Virol (2009)
- ATP and MO25α Regulate the Conformational State of the STRADα Pseudokinase and Activation of the LKB1 Tumour Suppressor – PLoS Biol (2009)
- Apratoxin A Reversibly Inhibits the Secretory Pathway by Preventing Cotranslational Translocation - Molecular Pharmacology (2009)
- NS4A regulates the ATPase activity of the NS3 helicase: a novel cofactor role of the non-structural protein NS4A from West Nile virus- Journal of General Virology (2009)
- Evidence That Two ATP-Dependent (Lon) Proteases in Borrelia burgdorferi Serve Different Functions - PLoS Pathogens (2009)
- ATP and MO25α Regulate the Conformational State of the STRADα Pseudokinase and Activation of the LKB1 Tumour Suppressor- PLoS Biology (2009)
- Stress-Dependent Coordination of Transcriptome and Translatome in Yeast - PLoS Biology (2009)
- An Afg2/Spaf-Related Cdc48-like AAA ATPase Regulates the Stability and Activity of the C. elegans Aurora B Kinase AIR-2 - Developmental Cell (2008)
- Staphylococcus aureus HrtA Is an ATPase Required for Protection against Heme Toxicity and Prevention of a Transcriptional Heme Stress Response - Journal of Bacteriology (2008)
- ATP scavenging by the intracellular pathogen Porphyromonas gingivalis inhibits P2X7-mediated host-cell apoptosis- Cell Microbiol. (2008)
- The Two-component NS2B-NS3 Proteinase Represses DNA Unwinding Activity of the West Nile Virus NS3 Helicase- JBC (2008)
- Functional characterization of the nucleotide binding domain of the Cryptosporidium parvum CpABC4 transporter: An iron–sulfur cluster transporter homolog- Molecular and Biochemical Parasitology (2008)
- Hippocampal levels of gamma-enolase, C-1-tetrahydrofolate synthase and serotransferrin fluctuate over the estrous cycle in the rat- Neuroscience (2008)
- Consumption of Soy Protein Isolate Modulates the Phosphorylation Status of Hepatic ATPase/ATP Synthase ß Protein and Increases ATPase Activity in Rats - J. Nutr. (2007)
- First identification and functional characterization of an immunogenic protein in unculturable haemotrophic Mycoplasmas (Mycoplasma suis HspA1)- FEMS Immunology & Medical Microbiology (2007)
- Identification, structure and mode of action of a new regulator of the Helicobacter pylori HP0525 ATPase - EMBO J. (2007)
- Proteome Profiling in the Rat Harderian Gland - J Proteome Res (2006)
【供應廠商】太鼎生物科技有限公司
【連絡人】許虹宜0920-312382
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【台中公司】陳俊豪 0935-229803
【公司網址】 www.biopioneer.com.tw
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