Angioreactor改善Plug assay所造成的問題。毎管體積20ul, 一隻mice可移植4管。萊恩生物科技有限公司 04-25239639

活體血管新生套組產品介紹 Directed In Vivo Angiogenesis Assay
1. 活體血管新生分析，市上唯一可定量血管新生的套組。
2. 改善plus assay所造成的自體吸收問題。
3. 20ul體積, 每隻mice可移植4管, 實驗再現性高。

DIVAA™ Background/Significance
• Invented and developed at the National Institute
of Health by William Stetler-Stevenson, MD,
Ph.D.
• Quantitative Assay to Assess Angiogenesis In
Vivo
• Alternative to Plug Assay
【血管新生分析------動物物種選擇】
Most data has been generated with athymic nude
mice, but success has been reported in C57Bl/6 as
well.
• Recommend 6 to 8 weeks old.
• Studies have been done with both males and
females. Females are recommended because males
may remove angioreactors from each other via
fighting.
【血管新生分析------埋殖準備】
• Optimal implantation periods may vary depending on
experimental design:
– angiogenic factors
– doses
– mice
• Genetic background
• Athymic nude mice (9-15 days)
• C57Bl/6 mice (15 days)
• Increasing implantation period generally improves
signal, but it may also increase background.
• Placement of the angioreactors is also critical.
Preparation

DIVAA Angiorack^™ Cat# 3450-048-09
Available as a separate product, the autoclavable, reusable Angiorack is precision machined of Teflon^® to hold 48 sterile silicone angioreactors in the laminar flow hood while filling and preparing for implantation.

Features:	This kit includes pathogen Free BME which has been tested and cleared of a Battery of 31 pathogens and viruses including LDEV. Contact Trevigen for details.
Applications:	Quantitative in vivo angiogenesis analysis, for the screening of inhibitors.
Components:	Part #	Component	Size
	3450-048-01	Angioreactors	48 units
	3450-048-02	Growth Factor Reduced BME PathClear	6 x 200 µl
	3450-048-03	Wash Buffer	25 ml
	3450-048-05	CellSperse™	15 ml
	3450-048-06	200X FITC-Lectin	250 µg/50 µl
	3450-048-07	25X FITC-Lectin Diluent	400 µl
	3450-048-08	Heparin Solution	10 µl
	3450-048-B10	FGF-2(1800 ng)/VEGF(600 ng)	60 µl
	*Basement Membrane Extract (BME)
Storage:	Components are stored at -20°C and 4°C.
References:	Guedez L, Rivera AM, Salloum R, Miller ML, Diegmueller JJ, Bungay PM, Stetler-Stevenson WG: Quantitative Assessment of Angiogenic Response by the Directed In Vivo Angiogenesis Assay. AJP 2003, pp 1431-1439.

詳細產品資訊，請登錄www.trevigen.com 查詢
 

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