GTPase分析套組---產品介紹
產品應用GTPases酵素活性之分析
內附有GTPase inhibitor。GTPase酵素活性分析，採用超高靈敏度Malachite green磷酸根呈色方法，克服傳統呈色方法,低靈敏度限制。測量目標樣本中， GTPases酵素活性。
GTPase活性分析檢測
產品應用
Innova Biosciences 提供之GTPase分析套組，可探討酵素活性是否扮演transport、signal transduction, protein biosynthesis及cell differentiation之角色。應用於GTPase活性分析。提供high-throughput screen 大量GTPase inhibitor。可建立快速藥物篩選系統。
GTPase活性分析檢測
產品特點
1. 提供high-throughput screen 大量GTPase inhibitor。
2. GTPase酵素分析套組，包括stabilizing solution，可避開acid labile substrate所造成的高背景值，其背景值很低(1mM )。
2. 其分析波段為600~660nm，避開一般colored compounds之干擾。
3. Pi-dye複合物極為穩定，其訊號強度可維持數小時之久。
4. Superior呈色系統。採用Malachite Green磷酸根呈色方式，呈色靈敏度比傳統呈色方法高，克服低靈敏度的限制。
5. 適用於大部份的assay buffer。
6. 蛋白質濃度高時，不會抑制顏色的形成。
訂購貨號# 602-0120, 196 assays (靈敏度4~40uM)
訂購貨號# 602-0121, 480 assays (靈敏度4~40uM)
訂購貨號# 602-0122, 480*5 assays (靈敏度4~40uM)
產品內容物
1 x 10ml of PiColorLock™ Gold* (1 x 25ml)
1 x 0.25ml of Accelerator (1 x 0.5ml)
1 x 5ml of Stabiliser (1 x 10ml)
1 x 1.5ml of 0.1M MgCl2 (2 x 1.5ml)
1 x 5ml of 0.5M Tris pH 7.5 (1 x 10ml)
1 x 5ml of 0.1mM Pi standard (1 x 10ml)
更多參考內容:
相關商品
PiColorLock磷酸根分析檢測套組
ATPase活性分析檢測套組
游離磷酸根移除resin
5g(501-0015)
Inorganic phosphate (Pi) is a product of many enzymatic reactions, for example, those catalysed by phosphatases and ATPases. Measurement of released Pi allows enzyme activity to be determined, but contamination of enzymes or buffers by Pi can lead to unacceptably high assay backgrounds.
PiBind™ resin provides a quick and easy way to remove contaminating Pi from buffers. The resin works over a broad range of pH values and is unaffected by many commonly used buffer additives. PiBind™ resin can also be used to remove Pi from protein samples (e.g. tissue extracts). This method is quicker than dialysis and unlike desalting does not necessarily lead to a significant dilution of the sample.
GTPase酵素活性分析套組---文獻發表
GTPase酵素活性分析套組
GTPase Assay Kit
- The selenocysteine-specific elongation factor contains a novel and multi-functional domain - The Journal of Biological Chemistry (2012)
- Tubulin nucleotide status controls Sas-4-dependent pericentriolar material recruitment - Nature Cell Biology (2012)
- The Mitochondrial Phosphatase PGAM5 Functions at the Convergence Point of Multiple Necrotic Death Pathways- Cell (2012)
- Leucyl-tRNA Synthetase Is an Intracellular Leucine Sensor for the mTORC1-Signaling Pathway - Cell (2012)
- ArfGAP1 Is a GTPase Activating Protein for LRRK2: Reciprocal Regulation of ArfGAP1 by LRRK2 - Journal of Neuroscience (2012)
- GTPase Activity and Neuronal Toxicity of Parkinson's Disease–Associated LRRK2 Is Regulated by ArfGAP1- PLoS Genetics (2012)
- AIP1 Functions as Arf6-GAP to Negatively Regulate TLR4 Signaling - JBC (2010)
- GTPase activity plays a key role in the pathobiology of LRRK2 - PLoS Genetics (2010)
- Role of the G-protein and tyrosine kinase—Rho/ROK pathways in 15-hydroxyeicosatetraenoic acid induced pulmonary vasoconstriction in hypoxic rats - J Biochem (2010)
- AIP1 Functions as Arf6-GAP to Negatively Regulate TLR4 Signaling - JBC (2010)
- Identification and Characterization of a Leucine-Rich Repeat Kinase 2 (LRRK2) Consensus Phosphorylation Motif- PLoS One (2010)
- Speckled-like Pattern in the Germinal Center (SLIP-GC), a Nuclear GTPase Expressed in Activation-induced Deaminase-expressing Lymphomas and Germinal Center B Cells- JBC (2009)
- High-level production and characterization of a G-protein coupled receptor signaling complex - FEBS Journal (2009)
- Structure of ERA in complex with the 3′ end of 16S rRNA: Implications for ribosome biogenesis- PNAS (2009)
- G Protein-Coupled Receptor-Promoted Trafficking of Gβ1γ2 Leads to AKT Activation at Endosomes via a Mechanism Mediated by Gβ1γ2-Rab11a Interaction – Molecular Biology of the Cell (2008)
- Characterization of Human GTPBP3, a GTP-Binding Protein Involved in Mitochondrial tRNA Modification - Molecular and Cellular Biology (2008)
- Comparative real-time PCR and enzyme analysis of selected gender-associated molecules in Schistosoma japonicum- Parasitology (2008)
【供應廠商】太鼎生物科技有限公司
【連絡人】許虹宜 0920-312382
【臺北公司】02-86609496
【台中公司】陳俊豪 0935-229803
【公司網址】 www.biopioneer.com.tw
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